Galactocerebrosidase (Galc), mouse, recombinant

Organism: Mus musculus (Mouse). Source: in vitro E.coli expression system. Expression Region: 43-684aa. Protein Length: Full Length of Mature Protein. Tag Info: Tag-Free. Target Protein Sequence: YVLDDSDGLG REFDGIGAVS GGGATSRLLV NYPEPYRSEI LDYLFKPNFG ASLHILKVEI GGDGQTTDGT EPSHMHYELD ENYFRGYEWW LMKEAKKRNP DIILMGLPWS FPGWLGKGFS WPYVNLQLTA YYVVRWILGA KHYHDLDIDY IGIWNERPFD ANYIKELRKM LDYQGLQRVR IIASDNLWEP ISSSLLLDQE LWKVVDVIGA HYPGTYTVWN AKMSGKKLWS SEDFSTINSN VGAGCWSRIL NQNYINGNMT STIAWNLVAS YYEELPYGRS GLMTAQEPWS GHYVVASPIW VSAHTTQFTQ PGWYYLKTVG HLEKGGSYVA LTDGLGNLTI IIETMSHQHS MCIRPYLPYY NVSHQLATFT LKGSLREIQE LQVWYTKLGT PQQRLHFKQL DTLWLLDGSG SFTLELEEDE IFTLTTLTTG RKGSYPPPPS SKPFPTNYKD DFNVEYPLFS EAPNFADQTG VFEYYMNNED REHRFTLRQV LNQRPITWAA DASSTISVIG DHHWTNMTVQ CDVYIETPRS GGVFIAGRVN KGGILIRSAT GVFFWIFANG SYRVTADLGG WITYASGHAD VTAKRWYTLT LGIKGYFAFG MLNGTILWKN VRVKYPGHGW AAIGTHTFEF AQFDNFRVEA AR. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Enzyme with very low activity responsible for the lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney and epithelial cells of small intestine and colon. Reference: "Molecular cloning and expression of cDNA for murine galactocerebrosidase and mutation analysis of the twitcher mouse, a model of Krabbe's disease."Sakai N., Inui K., Tatsumi N., Fukushima H., Nishigaki T., Taniike M., Nishimoto J., Tsukamoto H., Yanagihara I., Ozono K., Okada S.J. Neurochem. 66:1118-1124(1996). Function: Hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Enzyme with very low activity responsible for the lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney and epithelial cells of small intestine and colon.