Gag polyprotein (gag), partial, Moloney murine leukemia virus, recombinant

Organism: Moloney murine leukemia virus (isolate Shinnick) (MoMLV). Source: E.coli. Expression Region: 216-478aa. Protein Length: Partial. Tag Info: N-terminal GST-tagged. Target Protein Sequence: PLRAGGNGQL QYWPFSSSDL YNWKNNNPSF SEDPGKLTAL IESVLITHQP TWDDCQQLLG TLLTGEEKQR VLLEARKAVR GDDGRPTQLP NEVDAAFPLE RPDWDYTTQA GRNHLVHYRQ LLLAGLQNAG RSPTNLAKVK GITQGPNESP SAFLERLKEA YRRYTPYDPE DPGQETNVSM SFIWQSAPDI GRKLERLEDL KNKTLGDLVR EAEKIFNKRE TPEEREERIR RETEEKEERR RTEDEQKEKE RDRRRHREMS KLL. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release. Reference: Late domain-independent rescue of a release-deficient Moloney murine leukemia virus by the ubiquitin ligase Itch.Jadwin J.A., Rudd V., Sette P., Challa S., Bouamr F.J. Virol. 84:704-715(2010). Function: Gag polyprotein