Gag polyprotein (gag), partial, Feline immunodeficiency virus, recombinant

Organism: Feline immunodeficiency virus (isolate Petaluma) (FIV). Source: E.coli. Expression Region: 1-135aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: MGNGQGRDWK MAIKRCSNVA VGVGGKSKKF GEGNFRWAIR MANVSTGREP GDIPETLDQL RLVICDLQER REKFGSSKEI DMAIVTLKVF AVAGLLNMTV STAAAAENMY SQMGLDTRPS MKEAGGKEEG PPQAY. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Matrix protein p15 forms the outer shell of the core of the virus, lining the inner surface of the viral membrane., Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex., Nucleocapsid protein p13 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers. Reference: "Biophysical characterization and crystal structure of the Feline Immunodeficiency Virus p15 matrix protein." Serriere J., Robert X., Perez M., Gouet P., Guillon C. Retrovirology 10:64-64(2013). Function: nan