G-protein coupled receptor 62 (GPR62), human, recombinant

Organism: Homo sapiens (Human). Source: in vitro E.coli expression system. Expression Region: 1-368aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MANSTGLNAS EVAGSLGLIL AAVVEVGALL GNGALLVVVL RTPGLRDALY LAHLCVVDLL AAASIMPLGL LAAPPPGLGR VRLGPAPCRA ARFLSAALLP ACTLGVAALG LARYRLIVHP LRPGSRPPPV LVLTAVWAAA GLLGALSLLG TPPAPPPAPA RCSVLAGGLG PFRPLWALLA FALPALLLLG AYGGIFVVAR RAALRPPRPA RGSRLHSDSL DSRLSILPPL RPRLPGGKAA LAPALAVGQF AACWLPYGCA CLAPAARAAE AEAAVTWVAY SAFAAHPFLY GLLQRPVRLA LGRLSRRALP GPVRACTPQA WHPRALLQCL QRPPEGPAVG PSEAPEQTPE LAGGRSPAYQ GPPESSLS-. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Orphan G-protein coupled receptor. Constitutively activates the G(q/11)/inositol phosphate and the G(s)-alpha/cAMP signaling pathways . Has spontaneous activity for beta-arrestin recruitment . Shows a reciprocal modulation of signaling functions with the melatonin receptor MTNR1B most likely through receptor heteromerization . Reference: "Orphan GPR61, GPR62 and GPR135 receptors and the melatonin MT2 receptor reciprocally modulate their signaling functions." Oishi A., Karamitri A., Gerbier R., Lahuna O., Ahmad R., Jockers R. Sci. Rep. 7:8990-8990(2017). Function: nan