Frizzled-2 (FZD2), recombinant human

Organism: Homo sapiens (Human). Source: in vitro E.coli expression system. Expression Region: 24-565aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal10xHis-tagged. Target Protein Sequence: QFHGEKGISI PDHGFCQPIS IPLCTDIAYN QTIMPNLLGH TNQEDAGLEV HQFYPLVKVQ CSPELRFFLC SMYAPVCTVL EQAIPPCRSI CERARQGCEA LMNKFGFQWP ERLRCEHFPR HGAEQICVGQ NHSEDGAPAL LTTAPPPGLQ PGAGGTPGGP GGGGAPPRYA TLEHPFHCPR VLKVPSYLSY KFLGERDCAA PCEPARPDGS MFFSQEETRF ARLWILTWSV LCCASTFFTV TTYLVDMQRF RYPERPIIFL SGCYTMVSVA YIAGFVLQER VVCNERFSED GYRTVVQGTK KEGCTILFMM LYFFSMASSI WWVILSLTWF LAAGMKWGHE AIEANSQYFH LAAWAVPAVK TITILAMGQI DGDLLSGVCF VGLNSLDPLR GFVLAPLFVY LFIGTSFLLA GFVSLFRIRT IMKHDGTKTE KLERLMVRIG VFSVLYTVPA TIVIACYFYE QAFREHWERS WVSQHCKSLA IPCPAHYTPR MSPDFTVYMI KYLMTLIVGI TSGFWIWSGK TLHSWRKFYT RLTNSRHGET TV. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. Reference: n/a. Function: nan