Flotillin-1 (FLOT1), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 149-427aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: YTLKDIHDDQ DYLHSLGKAR TAQVQKDARI GEAEAKRDAG IREAKAKQEK VSAQYLSEIE MAKAQRDYEL KKAAYDIEVN TRRAQADLAY QLQVAKTKQQ IEEQRVQVQV VERAQQVAVQ EQEIARREKE LEARVRKPAE AERYKLERLA EAEKSQLIMQ AEAEAASVRM RGEAEAFAIG ARARAEAEQM AKKAEAFQLY QEAAQLDMLL EKLPQVAEEI SGPLTSANKI TLVSSGSGTM GAAKVTGEVL DILTRLPESV ERLTGVSISQ VNHKPLRTA. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. Reference: "A protein interaction network for Ecm29 links the 26 S proteasome to molecular motors and endosomal components." Gorbea C., Pratt G., Ustrell V., Bell R., Sahasrabudhe S., Hughes R.E., Rechsteiner M. J. Biol. Chem. 285:31616-31633(2010). Function: nan