Ficolin-1 (Fcn1), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 18-334aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: SQALGQERGA CPDVKVVGLG AQDKVVVIQS CPGFPGPPGP KGEPGSPAGR GERGFQGSPG KMGPAGSKGE PGTMGPPGVK GEKGDTGAAP SLGEKELGDT LCQRGPRSCK DLLTRGIFLT GWYTIHLPDC RPLTVLCDMD VDGGGWTVFQ RRVDGSIDFF RDWDSYKRGF GNLGTEFWLG NDYLHLLTAN GNQELRVDLQ DFQGKGSYAK YSSFQVSEEQ EKYKLTLGQF LEGTAGDSLT KHNNMSFTTH DQDNDANSMN CAALFHGAWW YHNCHQSNLN GRYLSGSHES YADGINWGTG QGHHYSYKVA EMKIRAS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Extracellular lectin functioning as a pattern-recognition receptor in innate immunity. Binds the sugar moieties of pathogen-associated molecular patterns (PAMPs) displayed on microbes and activates the lectin pathway of the complement system. May also activate monocytes through a G protein-coupled receptor, FFAR2, inducing the secretion of interleukin-8/IL-8. Binds preferentially to 9-O-acetylated 2-6-linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage. Reference: "Carbohydrate-binding specificities of mouse ficolin A, a splicing variant of ficolin A and ficolin B and their complex formation with MASP-2 and sMAP." Endo Y., Nakazawa N., Liu Y., Iwaki D., Takahashi M., Fujita T., Nakata M., Matsushita M. Immunogenetics 57:837-844(2005). Function: nan