Fibrinogen gamma chain (FGG), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 27-453aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: YVATRDNCCI LDERFGSYCP TTCGIADFLS TYQTKVDKDL QSLEDILHQV ENKTSEVKQL IKAIQLTYNP DESSKPNMID AATLKSRKML EEIMKYEASI LTHDSSIRYL QEIYNSNNQK IVNLKEKVAQ LEAQCQEPCK DTVQIHDITG KDCQDIANKG AKQSGLYFIK PLKANQQFLV YCEIDGSGNG WTVFQKRLDG SVDFKKNWIQ YKEGFGHLSP TGTTEFWLGN EKIHLISTQS AIPYALRVEL EDWNGRTSTA DYAMFKVGPE ADKYRLTYAY FAGGDAGDAF DGFDFGDDPS DKFFTSHNGM QFSTWDNDND KFEGNCAEQD GSGWWMNKCH AGHLNGVYYQ GGTYSKASTP NGYDNGIIWA TWKTRWYSMK KTTMKIIPFN RLTIGEGQQH HLGGAKQVRP EHPAETEYDS LYPEDDL. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Together with fibrinogen alpha and fibrinogen beta, polymerizes to form an insoluble fibrin matrix. Has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways. Reference: "Nucleotide sequence of the gene for the gamma chain of human fibrinogen." Rixon M.W., Chung D.W., Davie E.W. Biochemistry 24:2077-2086(1985). Function: nan