Fc (mouse) IgG2a Control (rec.) (non-lytic)

Source/Host: HEK 293 cells Sequence: Fc (mouse) IgG2a (100-330 including 4 mutations). Crossreactivity: Mouse Application: Negative Control MW: ~28kDa (SDS-PAGE) Purity: >95% (SDS-PAGE) Endotoxin Content: <0.01EU/µg purified protein (LAL test). Concentration: After reconstitution: for 10µg size: 0.1mg/ml for 100µg size: 1mg/ml Reconstitution: Reconstitute with 100µl endotoxin-free water. Formulation: Lyophilized. Contains PBS. Negative control in experiments with recombinant proteins fused to Fc (mouse IgG2a (non-lytic) (e.g. Prod. No. AG-40B-0231). This protein is not an appropriate negative control for other standard Fc protein constructs. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the non-lytic proteins incapable of antibody-directed cytotoxicity (ADCC) and complement-directed cytotoxicity (CDC).