FAD synthase (FLAD1), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-490aa. Protein Length: Full Length of Isoform 2. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MTSRASELSP GRSVTAGIII VGDEILKGHT QDTNTFFLCR TLRSLGVQVC RVSVVPDEVA TIAAEVTSFS NRFTHVLTAG GIGPTHDDVT FEAVAQAFGD ELKPHPKLEA ATKALGGEGW EKLSLVPSSA RLHYGTDPCT GQPFRFPLVS VRNVYLFPGI PELLRRVLEG MKGLFQNPAV QFHSKELYVA ADEASIAPIL AEAQAHFGRR LGLGSYPDWG SNYYQVKLTL DSEEEGPLEE CLAYLTARLP QGSLVPYMPN AVEQASEAVY KLAESGSSLG KKVAGALQTI ETSLAQYSLT QLCVGFNGGK DCTALLHLFH AAVQRKLPDV PNPLQILYIR SISPFPELEQ FLQDTIKRYN LQMLEAEGSM KQALGELQAR HPQLEAVLMG TRRTDPYSCS LCPFSPTDPG WPAFMRINPL LDWTYRDIWD FLRQLFVPYC ILYDRGYTSL GSRENTVRNP ALKCLSPGGH PTYRPAYLLE NEEEERNSRT. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme. Reference: Cloning, expression and characterization of a human FAD synthetase.Fischer M.J., Kempter K., Bacher A. Chen X.G., Li Y. Large-scale cDNA transfection screening for genes related to cancer development and progression.Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004). Function: Catalyzes the adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme.