Exonuclease III

Product Description: Exonuclease III is a double-stranded DNA-specific exonuclease. Exonuclease III catalyzes the removal of nucleotides from linear or nicked double stranded DNA in the 3' to 5' direction. The degradation of Exonuclease III could be initiated from 3' blunt end, 3' recessed end, 3' overhangs with less than 4 bases and nicked DNA. Application: Site-directed mutagenesis, Preparation of nested deletions in dsDNA, Preparation of strand-specific probes, Preparation of ssDNA for dideoxy-sequencing. Concentration: 100U/µL. Unit Definition: One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble total nucleotides in 30 minutes at 37°C in a total reaction volume of 50µL. Source: E. coli strain that carries E. coli Exonuclease III gene.