Eukaryotic initiation factor 4A-I (EIF4A1), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-406aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: SASQDSRSRD NGPDGMEPEG VIESNWNEIV DSFDDMNLSE SLLRGIYAYG FEKPSAIQQR AILPCIKGYD VIAQAQSGTG KTATFAISIL QQIELDLKAT QALVLAPTRE LAQQIQKVVM ALGDYMGASC HACIGGTNVR AEVQKLQMEA PHIIVGTPGR VFDMLNRRYL SPKYIKMFVL DEADEMLSRG FKDQIYDIFQ KLNSNTQVVL LSATMPSDVL EVTKKFMRDP IRILVKKEEL TLEGIRQFYI NVEREEWKLD TLCDLYETLT ITQAVIFINT RRKVDWLTEK MHARDFTVSA MHGDMDQKER DVIMREFRSG SSRVLITTDL LARGIDVQQV SLVINYDLPT NRENYIHRIG RGGRFGRKGV AINMVTEEDK RTLRDIETFY NTSIEEMPLN VADLI. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. Reference: "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S. Nat. Genet. 36:40-45(2004). Function: nan