Epoxide hydrolase 3 (EPHX3), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 43-360aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: ALTHVLCRPR RGCCGRRRSA SPACLSDPSL GEHGFLNLKS SGLRLHYVSA GRGNGPLMLF LHGFPENWFS WRYQLREFQS RFHVVAVDLR GYGPSDAPRD VDCYTIDLLL VDIKDVILGL GYSKCILVAH DWGALLAWHF SIYYPSLVER MVVVSGAPMS VYQDYSLHHI SQFFRSHYMF LFQLPWLPEK LLSMSDFQIL KTTLTHRKTG IPCLTPSELE AFLYNFSQPG GLTGPLNYYR NLFRNFPLEP QELTTPTLLL WGEKDTYLEL GLVEAIGSRF VPGRLEAHIL PGIGHWIPQS NPQEMHQYMW AFLQDLLD. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the hydrolysis of epoxide-containing fatty acids. Active in vitro against epoxyeicosatrienoic acids (EETs) including 8,9-EET, 9,10-EET, 11,12-EET and 14,15-EET and leukotoxin. Reference: "EH3 (ABHD9): the first member of a new epoxide hydrolase family with high activity for fatty acid epoxides." Decker M., Adamska M., Cronin A., Di Giallonardo F., Burgener J., Marowsky A., Falck J.R., Morisseau C., Hammock B.D., Gruzdev A., Zeldin D.C., Arand M. J. Lipid Res. 53:2038-2045(2012). Function: nan