EphA2, Recombinant, Human, N-terminal His tag (Ephrin A2)

Human EPHA2 (GenBank Accession No. NM_004431), (a.a. 596-end) with N-terminal His tag. MW 47kD, expressed in an Baculovirus infected Sf9 cell expression system. , The Eph receptors are the largest family of tyrosine kinases. They can be grouped into two subclasses based upon sequence homology: the EphA and EphB receptors. The ligands for the Eph receptors are the ephrins. The EphA receptors bind ephrin-A ligands, which are tethered to the membrane by a GPI linkage, and the EphB receptors bind ephrin-B lignads, which have a membrane spanning region. The Eph receptors are widely expressed during embryonic development, including in the nervous and vascular systems. Ephrins and Eph receptors have been implicated in many developmental processes, including neuronal network formation, guidance of cell migration and axonal pathfinding. They remain expressed at lower levels in adult tissues, and are upregulated in pathological conditions such as cancer. , EphA2 has been previously named Eck, Myk2 and Sek2. It binds to ephrin-A1, -A3, -A4 and -A5, and is expressed most highly in tissues that contain a high proportion of epithelial cells, e.g. skin, intestine, lung and ovary. Purity: >80% by SDS-PAGE. Specific Activity: 170 U/mg. One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to tyrosine substrate per minute at pH 7.4 and 30°C. , Assay buffer: 50mM HEPES, pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3uM Na-orthovanadate, 0.5mM ATP, 30ug/ml Poly (Glu:Tyr)4:1 substrate, and 0.1ug /ml recombinant EPHA2. Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. , Storage and Stability:, Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.