Cookie preferences
This website uses cookies, which are necessary for the technical operation of the website and are always set. Other cookies, which increase the comfort when using this website, are used for direct advertising or to facilitate interaction with other websites and social networks, are only set with your consent.
Configuration
Technically required
These cookies are necessary for the basic functions of the shop.
"Allow all cookies" cookie
"Decline all cookies" cookie
CSRF token
Cookie preferences
Currency change
Customer-specific caching
FACT-Finder tracking
Individual prices
Selected shop
Session
Comfort functions
These cookies are used to make the shopping experience even more appealing, for example for the recognition of the visitor.
Note
Show the facebook fanpage in the right blod sidebar
Statistics & Tracking
Affiliate program
Conversion and usertracking via Google Tag Manager
Track device being used
Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
Cay11868-10 | 10 U | - |
6 - 10 business days* |
571.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Endothelial nitric oxide synthase (eNOS), also known as NOS3, converts arginine to citrulline and... more
Product information "eNOS (bovine, recombinant, E. coli expressed)"
Endothelial nitric oxide synthase (eNOS), also known as NOS3, converts arginine to citrulline and nitric oxide (NO) to regulate endothelial homeostasis and vascular tone. It is composed of an N-terminal oxygenase domain that contains binding sites for heme, its substrate L-arginine (Cay-23703), and the cofactor tetrahydrobiopterin (BH4) and a reductase domain with binding sites for NADPH, flavin mononucleotide, flavin-adenine dinucleotide, and calmodulin. eNOS is expressed in endothelial cells, as well as blood cells and circulating microparticles, with expression levels changing in response to growth factors, cytokines, and mechanical stimulation. Localization of eNOS is mediated by protein fatty acid acylation, with myristoylated and palmitoylated eNOS targeted to plasma membrane caveolae and the Golgi complex. eNOS functions as a homodimer and is activated by the binding of calcium-bound calmodulin. eNOS uncoupling and dysfunction, primarily induced by the loss of BH4 due to oxidation or decreased expression of dihydrofolate reductase (DHFR), induces the production of superoxide in place of NO and is a hallmark of most cardiovascular diseases, including atherosclerosis. Cayman's eNOS (bovine, recombinant, E. coli expressed) can be used for enzyme assay applications.Synonyms: Constitutive NOS, cNOS, Endothelial Nitric Oxide Synthase, Endothelial NOS, Nitric Oxide Synthase 3. Purity: Partially pure. Protein Identity Confirmed by Mass Spec: Yes. Source: Active recombinant bovine N-terminal His-tagged eNOS expressed in E. coli. Amino Acids: 2-1,205 (full length). MW: 134 kDa. Formulation: (Request formulation change), 50 mM HEPES, pH 7.8, with 0.5 mM DTT, 0.5 mM EDTA, 300 mM sodium chloride, 2 mM calcium chloride, 10 µM tetrahydro-L-biopterin (hydrochloride), and 20% glycerol. UniProt Accession #: P29473.
Keywords: | NOS3, cNOS, eNOS, cNOS, eNOS, EC-NOS, NOSIII, EC-NOS, NOSIII, NOS type III, NOS type III, Endothelial NOS, Endothelial NOS, Constitutive NOS, Constitutive NOS, Nitric oxide synthase, endothelial, Nitric oxide synthase, endothelial |
Supplier: | Cayman Chemical |
Supplier-Nr: | 11868 |
Properties
Application: | Enzyme activity assay |
Conjugate: | No |
Purity: | Partially pure |
Format: | Partially Purified |
Database Information
KEGG ID : | K13242 | Matching products |
UniProt ID : | P29473 | Matching products |
Handling & Safety
Storage: | -80°C |
Shipping: | -80°C (International: -80°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
more
You will get a certificate here
Viewed