Endoglycosylceramidase 5A, Rhodococcus sp.

Endoglycosylceramidase 5A (REgc5A), assigned the E.C. number 3.2.1.123, is a derivative of Rhodococcus sp. It is an endoglycosylceramidase. The recombinant REgc5A, purified from Escherichia coli, is a single-domain Glycoside Hydrolase family 5 (GH5) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 0,25 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Glycoside Hydrolase. Activity: Other Activities. Family: GH5. Ec Number: 3.2.1.123. Organism: Rhodococcus sp. Architecture: GH5. Optimal pH: 4.5. Optimal Temperature: 37°C. Substrates: the glycosidic linkage between oligosaccharides and ceramides of various glycosphingolipids