Endogenous retrovirus group K member 7 Env polyprotein (ERVK-7), human, recombinant

Organism: Homo sapiens (Human). Source: in vitro E.coli expression system. Expression Region: 1-588aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MVTPVTWMDN PIEIYVNDSV WVPGPIDDRC PAKPEEEGMM INISIGYRYP PICLGRAPGC LMPAVQNWLV EVPTVSPISR FTYHMVSGMS LRPRVNYLQD FSYQRSLKFR PKGKPCPKEI PKESKNTEVL VWEECVANSA VILQNNEFGT IIDWAPRGQF YHNCSGQTQS CPSAQVSPAV DSDLTESLDK HKHKKLQSFY PWEWGEKRIS TPRPKIVSPV SGPEHPELWR LTVASHHIRI WSGNQTLETR DCKPFYTIDL NSSLTVPLQS CVKPPYMLVV GNIVIKPDSQ TITCENCRLL SCIDSTFNWQ HRILLVRARE GVWIPVSMDR PWEASPSVHI LTEVLKGVLN RSKRFIFTLI AVIMGLIAVT ATAAVAGVAL HSSVQSVNFV NDWQKNSTRL WNSQSSIDQK LANQINDLRQ TVIWMGDRLM SLEHRFQLQC DWNTSDFCIT PQIYNESEHH WDMVRRHLQG REDNLTLDIS KLKEQIFEAS KAHLNLVPGT EAIAGVADGL ANLNPVTWVK TIGSTTIINL ILILVCLFCL LLVCRCTQQL RRDSDHRERA MMTMAVLSKR KGGNVGKSKR DQIVTVSV. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution., SU mediates receptor recognition. TM anchors the envelope heterodimer to the viral membrane through one transmembrane domain. The other hydrophobic domain, called fusion peptide, mediates fusion of the viral membrane with the target cell membrane . Reference: "Transcriptionally active HERV-K genes: identification, isolation, and chromosomal mapping." Sugimoto J., Matsuura N., Kinjo Y., Takasu N., Oda T., Jinno Y. Genomics 72:137-144(2001). Function: nan