ECSIT, Recombinant, Human, aa19-217, His-Tag (Evolutionarily Conserved Signaling Intermediate in Tol

ECSIT is a ubiquitously expressed protein that plays an important role as an adaptor protein in the cytosolic signal transduction cascade events triggered by Toll receptor activation. It was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway. Knockdown of ECSIT results in severely impaired complex I assembly and disturbed mitochondrial function. Source: Recombinant protein corresponding to aa19-217 from human ECSIT at N-terminal, fused to His-tag expressed in E.coli. Molecular Weight: ~24.6kD (222aa), AA Sequence: MGSSHHHHHH SSGLVPRGSH MGS VYYHILRADL LPPEEREVEE TPEEWNLYYP MQLDLEYVRS GWDNYEFDIN EVEEGPVFAM CMAGAHDQAT MAKWIQGLQE TNPTLAQIPV VFRLAGSTRE LQTSSAGLEE PPLPEDHQEE DDNLQRQQQG QS GTCGAAL TGTSISQVPL PKDSTGAADP PQPHIVGIQS PDQQAALARH NPARPVFVEG PFSLWLRNKC, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.