E3 ubiquitin-protein ligase HUWE1 (HUWE1), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 4005-4374aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: LERLDEGLRK EDMAVHVRRD HVFEDSYREL HRKSPEEMKN RLYIVFEGEE GQDAGGLLRE WYMIISREMF NPMYALFRTS PGDRVTYTIN PSSHCNPNHL SYFKFVGRIV AKAVYDNRLL ECYFTRSFYK HILGKSVRYT DMESEDYHFY QGLVYLLEND VSTLGYDLTF STEVQEFGVC EVRDLKPNGA NILVTEENKK EYVHLVCQMR MTGAIRKQLA AFLEGFYEII PKRLISIFTE QELELLISGL PTIDIDDLKS NTEYHKYQSN SIQIQWFWRA LRSFDQADRA KFLQFVTGTS KVPLQGFAAL EGMNGIQKFQ IHRDDRSTDR LPSAHTCFNQ LDLPAYESFE KLRHMLLLAI QECSEGFGLA. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Ubiquitinates MFN2 to negatively regulate mitochondrial fusion in response to decreased stearoylation of TFRC. Ubiquitination of MFN2 also takes place following induction of mitophagy, AMBRA1 acts as a cofactor for HUWE1-mediated ubiquitination. Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation. Mediates polyubiquitination of isoform 2 of PA2G4. Acts in concert with MYCBP2 to regulate the circadian clock gene expression by promoting the lithium-induced ubiquination and degradation of NR1D1. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Reference: "HUWE1 E3 ligase promotes PINK1/PARKIN-independent mitophagy by regulating AMBRA1 activation via IKKalpha." Di Rita A., Peschiaroli A., D'Acunzo P., Strobbe D., Hu Z., Gruber J., Nygaard M., Lambrughi M., Melino G., Papaleo E., Dengjel J., El Alaoui S., Campanella M., Doetsch V., Rogov V.V., Strappazzon F., Cecconi F. Nat. Commun. 9:3755-3755(2018). Function: nan