E3 ubiquitin-protein ligase DTX3L (DTX3L), recombinant human

Organism: Homo sapiens (Human). Source: Yeast. Expression Region: 2-740aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal Twin-Strep-tagged. Target Protein Sequence: ASHLRPPSPL LVRVYKSGPR VRRKLESYFQ SSKSSGGGEC TVSTQEHEAP GTFRVEFSER AAKERVLKKG EHQILVDEKP VPIFLVPTEN SIKKNTRPQI SSLTQSQAET PSGDMHQHEG HIPNAVDSCL QKIFLTVTAD LNCNLFSKEQ RAYITTLCPS IRKMEGHDGI EKVCGDFQDI ERIHQFLSEQ FLESEQKQQF SPSMTERKPL SQQERDSCIS PSEPETKAEQ KSNYFEVPLP YFEYFKYICP DKINSIEKRF GVNIEIQESS PNMVCLDFTS SRSGDLEAAR ESFASEFQKN TEPLKQECVS LADSKQANKF KQELNHQFTK LLIKEKGGEL TLLGTQDDIS AAKQKISEAF VKIPVKLFAA NYMMNVIEVD SAHYKLLETE LLQEISEIEK RYDICSKVSE KGQKTCILFE SKDRQVDLSV HAYASFIDAF QHASCQLMRE VLLLKSLGKE RKHLHQTKFA DDFRKRHPNV HFVLNQESMT LTGLPNHLAK AKQYVLKGGG MSSLAGKKLK EGHETPMDID SDDSKAASPP LKGSVSSEAS ELDKKEKGIC VICMDTISNK KVLPKCKHEF CAPCINKAMS YKPICPTCQT SYGIQKGNQP EGSMVFTVSR DSLPGYESFG TIVITYSMKA GIQTEEHPNP GKRYPGIQRT AYLPDNKEGR KVLKLLYRAF DQKLIFTVGY SRVLGVSDVI TWNDIHHKTS RFGGPEMYGY PDPSYLKRVK EELKAKGIE. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: E3 ubiquitin-protein ligase which, in association with ADP-ribosyltransferase PARP9, plays a role in DNA damage repair and in interferon-mediated antiviral responses. Reference: n/a. Function: nan