Dystroglycan (DAG1), partial, rabbit, recombinant

Organism: Oryctolagus cuniculus (Rabbit). Source: Mammalian cell. Expression Region: 30-653aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: HWPSEPSEAV RDWENQLEAS MHSVLSDLHE ALPTVVGIPD GTAVVGRSFR VTIPTDLIGS SGEVIKVSTA GKEVLPSWLH WDPQSHTLEG LPLDTDKGVH YISVSAAQLD ANGSHIPQTS SVFSIEVYPE DHSEPQSVRA ASPDLGEAAA SACAAEEPVT VLTVILDADL TKMTPKQRID LLHRMQSFSE VELHNMKLVP VVNNRLFDMS AFMAGPGNAK KVVENGALLS WKLGCSLNQN SVPDIRGVEA PAREGTMSAQ LGYPVVGWHI ANKKPPLPKR IRRQIHATPT PVTAIGPPTT AIQEPPSRIV PTPTSPAIAP PTETMAPPVR DPVPGKPTVT TRTRGAIIQT PTLGPIQPTR VSDAGTVVSG QIRATVTIPG YVEPTAVATP PTTTTKKPRV STPKPATPST DSSATTTRRP TKKPRTPRPV PRVTTKAPIT RLETASPPTR IRTTTSGVPR GGEPNQRPEL KNHIDRVDAW VGTYFEVKIP SDTFYDKEDT TTDKLKLTLK LREQQLVGEK SWVQFNSNSQ LMYGLPDSSH VGKHEYFMHA TDKGGLSAVD AFEIHVHKRP QGDKAPARFK AKFVGDPAPV VNDIHKKIAL VKKLAFAFGD RNCSTVTLQN ITRG. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: The dystroglycan complex is involved in a number of processes including laminin and basement membrane assembly, sacrolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Alpha-dystroglycan is an extracellular peripheral glycoprotein that acts as a receptor for both extracellular matrix proteins containing laminin-G domains. Receptor for laminin-2 (LAMA2) and agrin in peripheral nerve Schwann cells. Beta-dystroglycan is a transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton. Acts as a cell adhesion receptor in both muscle and non-muscle tissues. Receptor for both DMD and UTRN and, through these interactions, scaffolds axin to the cytoskeleton. Also functions in cell adhesion-mediated signaling and implicated in cell polarity (By similarity). Reference: 2 "Detection of O-mannosyl glycans in rabbit skeletal muscle alpha-dystroglycan." Sasaki T., Yamada H., Matsumura K., Shimizu T., Kobata A., Endo T. Biochim. Biophys. Acta 1425:599-606(1998). Function: nan