Dolichyl-phosphate-mannose--protein mannosyltransferase 3 (PMT3), partial, Saccharomyces cerevisiae,

Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). Source: E.coli. Expression Region: 304-602aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: LLSNSGPGDS TMPSLFQASL NGTKVGKGPR DVALGSSIIS IKNQALGGAL LHSHVQPFPE GSEQQQVTVY GYSDANNEWF FQRIRGVEPW TDAENKTIEF VKGGEMYRLM HRLTGKNLHT HEVPAPISKS EYEVSAYGDV DLGDYKDNWI IEIVEQVGEE DPTLLHPLST SFRIKNSILG CYLAQSGKHL PEWGFRQGEV VCLKHASKRD KRTWWNIETH ENERLPQGED FVYPKTSFFR NFMQLNSAMM ATNNALVPNP EKFDGIASSA WQWPTLNVGV RLCEWSEKSI KYFLLGSPA. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Protein O-mannosyltransferase involved in O-glycosylation which is essential for cell wall rigidity. Forms a heterodimeric complex with PMT5 and more rarely with PMT1 to transfer mannose from Dol-P-mannose to Ser or Thr residues on proteins. Seems to have redundant activity to PMT2. Reference: "PMT3 and PMT4, two new members of the protein-O-mannosyltransferase gene family of Saccharomyces cerevisiae." Immervoll T., Gentzsch M., Tanner W. Yeast 11:1345-1351(1995). Function: nan