DNase I

Deoxyribonuclease I (DNase I) is DNA-specific endonuclease that cleaves both single-stranded DNA,double-stranded DNA and DNA-RNA hybrids, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3' producing tetranucleotides. The activity of DNase I is strictly dependent on Ca2+ and can be activated by divalent metal ions such as Mg2+ or Mn2+. In the presence of Mg2+, DNase I nonspecifically recognizes and cleaves a double-stranded DNA at anysite on either strand, and in the presence of Mn2+, it recognizes and cleaves almost the same sites on both strands of the DNA to produce DNA fragments with blunt ends or sticky ends with 1~2 nucleotide overhangs. GenScript is offering DNase I produced by expression in a P. pastoris strain carrying a plasmid encoding the bovine DNase I.