DNA repair and recombination protein RAD52 (RAD52), partial, Saccharomyces cerevisiae, recombinant

Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). Source: E.coli. Expression Region: 60-282aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: IFGYNGWSTE VKSVVIDFLD ERQGKFSIGC TAIVRVTLTS GTYREDIGYG TVENERRKPA AFERAKKSAV TDALKRSLRG FGNALGNCLY DKDFLAKIDK VKFDPPDFDE NNLFRPTDEI SESSRTNTLH ENQEQQQYPN KRRQLTKVTN TNPDSTKNLV KIENTVSRGT PMMAAPAEAN SKNSSNKDTD LKSLDASKQD QDDLLDDSLM FSDDFQDDDL INM. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Involved in DNA double-strand break (DSB) repair and recombination. Promotes the annealing of complentary single-stranded DNA and by stimulation of the RAD51 recombinase. Reference: A multidimensional chromatography technology for in-depth phosphoproteome analysis.Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.Mol. Cell. Proteomics 7:1389-1396(2008). Function: Involved in DNA double-strand break (DSB) repair and recombination. Promotes the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.