DNA polymerase I, thermostable (polA),partial, Thermus aquaticus, recombinant

Organism: Thermus aquaticus. Source: Yeast. Expression Region: 289-832aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: ESPKALEEAP WPPPEGAFVG FVLSRKEPMW ADLLALAAAR GGRVHRAPEP YKALRDLKEA RGLLAKDLSV LALREGLGLP PGDDPMLLAY LLDPSNTTPE GVARRYGGEW TEEAGERAAL SERLFANLWG RLEGEERLLW LYREVERPLS AVLAHMEATG VRLDVAYLRA LSLEVAEEIA RLEAEVFRLA GHPFNLNSRD QLERVLFDEL GLPAIGKTEK TGKRSTSAAV LEALREAHPI VEKILQYREL TKLKSTYIDP LPDLIHPRTG RLHTRFNQTA TATGRLSSSD PNLQNIPVRT PLGQRIRRAF IAEEGWLLVA LDYSQIELRV LAHLSGDENL IRVFQEGRDI HTETASWMFG VPREAVDPLM RRAAKTINFG VLYGMSAHRL SQELAIPYEE AQAFIERYFQ SFPKVRAWIE KTLEEGRRRG YVETLFGRRR YVPDLEARVK SVREAAERMA FNMPVQGTAA DLMKLAMVKL FPRLEEMGAR MLLQVHDELV LEAPKERAEA VARLAKEVME GVYPLAVPLE VEVGIGEDWL SAKE. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: n/a. Reference: "Overproduction of Thermus aquaticus DNA polymerase and its structural analysis by ion-spray mass spectrometry." Ishino Y., Ueno T., Miyagi M., Uemori T., Imamura M., Tsunasawa S., Kato I. J. Biochem. 116:1019-1024(1994). Function: nan