Disintegrin and metalloproteinase domain-containing protein 12 (Adam12), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: Baculovirus. Expression Region: 206-706aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: ETLKMTKYVE LVIVADNREF QRQGKDLEKV KQRLIEIANH VDKFYRPLNI RIVLVGVEVW NDIDKCSISQ DPFTSLHEFL DWRKIKLLPR KSHDNAQLIS GVYFQGTTIG MAPIMSMCTA EQSGGVVMDH SDSPLGAAVT LAHELGHNFG MNHDTLERGC SCRMAAEKGG CIMNPSTGFP FPMVFSSCSR KDLEASLEKG MGMCLFNLPE VKQAFGGRKC GNGYVEEGEE CDCGEPEECT NRCCNATTCT LKPDAVCAHG QCCEDCQLKP PGTACRGSSN SCDLPEFCTG TAPHCPANVY LHDGHPCQGV DGYCYNGICQ THEQQCVTLW GPGAKPAPGI CFERVNSAGD PYGNCGKDSK SAFAKCELRD AKCGKIQCQG GASRPVIGTN AVSIETNIPQ QEGGRILCRG THVYLGDDMP DPGLVLAGTK CAEGKICLNR RCQNISVFGV HKCAMQCHGR GVCNNRKNCH CEAHWAPPFC DKFGFGGSTD SGPIRQADNQ G. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. Reference: "Spatially- and temporally-restricted expression of meltrin alpha (ADAM12) and beta (ADAM19) in mouse embryo." Kurisaki T., Masuda A., Osumi N., Nabeshima Y., Fujisawa-Sehara A. Mech. Dev. 73:211-215(1998). Function: Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.