Desmoglein-3 (Dsg3), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 50-617aa. Protein Length: Extracellular Domain. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: EWVKFAKPCR EREDNSRRNP IAKITSDFQK NQKITYRISG VGIDQPPFGI FVVDPNNGDI NITAIVDREE TPSFLITCRA LNALGQDVER PLILTVKILD VNDNPPIFSQ TIFKGEIEEN SASNSLVMIL NATDADEPNH MNSKIAFKIV SQEPAGMSMF LISRNTGEVR TLTSSLDREQ ISSYHLVVSG ADNDGTGLST QCECSIKIKD VNDNFPVLRE SQYSARIEEN TLNAELLRFQ VTDWDEEYTD NWLAVYFFTS GNEGNWFEIE TDPRTNEGIL KVVKALDYEQ VQSMQFSIAV RNKAEFHQSV ISQYRVQSTP VTIQVIDVRE GISFRPPSKT FTVQRGVSTN KLVGYILGTY QATDEDTGKA ASSVRYVLGR NDGGLLVIDS KTAQIKFVKN IDRDSTFIVN KTISAEVLAI DENTGKTSTG TIYVEVPSFN ENCPSVVLEK KDICTSSPSV TLSVRTLDRG KYTGPYTVSL EEQPLKLPVM WTITTLNATS ALLQAQQQVS PGVYNVPVIV KDNQDGLCDT PESLTLTVCQ CDDRSMCRAP IPSREPNTYG ESSWRLGP. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. Reference: "cDNA cloning and chromosomal assignment of the mouse gene for desmoglein 3 (Dsg3), the pemphigus vulgaris antigen."Ishikawa H., Silos S.A., Tamai K., Copeland N.G., Gilbert D.J., Jenkins N.A., Uitto J.Mamm. Genome 5:803-804(1994). Function: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.