Deoxyribonuclease II (DNase II ) (EC 3.1.22.1, 5'-Oligonucleotidohydrolase)

Deoxyribonuclease II is a glycoprotein endonuclease with a dimeric structure. Deoxyribonuclease II from porcine spleen has a molecular weight of 38kD. Optimum pH range is 4.5-5.0 at ionic strength 0.15M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II. Applications: DNA Sequencing (acidic pH), DNA Digestion (acidic pH), Activity: As Reported, % Protein: As Reported, Unit Definition: 1 unit causes an increase in absorbance at 260nm of 0.001 per minute per minute at 25ºC, pH 4.6 using highly polymerized DNA as substrate. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.