Deoxyribonuclease I (DNase I), 1000-1500 U/mg, lyophilized

Endonuclease which digests dsDNA by hydrolysis of deoxyribonucleotide linkages, producing 3'-hydroxyl oligonucleotides. Supplied as a chromatographically purified, lyophilized powder. Applications: Nick Translation, DNase I DNA Footprinting, Cloning of Random DNA Fragments, Source: Bovine pancreas, Form: Supplied as a chromatographically purified, salt-free, off-white lyophilized powder. Activity: >1500u/mg, Unit Definition: 1 unit causes an increase in absorbance at 260nm of 0.001 per minute per ml at 25ºC when acting upon highly polymerized DNA at pH 5.0. , Note: 0.005 Kunitz units digests 1ug DNA in 10 minutes at 37°C, 50mM Tris, 1mM Ca+2, 1mM Mg+2 in a 50ul reaction. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Certificate of Origin: The raw animal product used in the manufacturing of Deoxyribonuclease I (DNase I) is derived from animal material originating in New Zealand, Uruguian or Brazil where there have been no reported cases of bovine spongiform encephalopathy (BSE). During processing, the product is subjected to a pH of 1.8 for a minimum period of 14 hours.