DCAF5/DDB1/DDA1/Rbx1/CUL4B Complex Recombinant

Recombinant complex of full length human DCAF5 (DDB1 and CUL4 associated factor 5), amino acids 2-942(end), DBB1 (damage specific DNA binding protein 1), amino acids 2-1140(end), DDA1 (DET1 and DDB1 associated 1), amino acids 2-102(end), Rbx1 (ring-box 1), amino acids 2-108(end), and CUL4B (cullin 4B), amino acids 3-913(end). DCAF5, DBB1, and DDA1 contain an N-terminal FLAG-tag. Rbx1 contains the M5I mutation and contains an N-terminal His-tag (6xHis). CUL4B contains an N-terminal His-tag (6xHis). These recombinant proteins are co-expressed in a HEK293 expression system and affinity purified as a complex.