DCAF1/Rbx1 (M5I)/DDB1/CUL4A Complex

Complex of recombinant human full length DCAF1 (DDB1 and CUL4 Associated Factor 1), amino acids 2-1507(end), full length Rbx1 (ring-box 1), amino acids 2-108(end), full length DDB1 (damage specific DNA binding protein 1), amino acids 2-1140(end) and full length CUL4A (cullin 4A), amino acids 2-759(end). The DCAF1 and DDB1 constructs include an N-terminal FLAG-tag. The Rbx1 and CUL4 constructs contain an N-terminal His-tag (6xHis). The Rbx construct also includes the mutation M5I. These recombinant proteins were co-expressed, and affinity purified.