D-amino-acid oxidase (Dao), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 1-345aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MRVAVIGAGV IGLSTALCIH ERYHPTQPLH MKIYADRFTP FTTSDVAAGL WQPYLSDPSN PQEAEWSQQT FDYLLSCLHS PNAEKMGLAL ISGYNLFRDE VPDPFWKNAV LGFRKLTPSE MDLFPDYGYG WFNTSLLLEG KSYLPWLTER LTERGVKLIH RKVESLEEVA RGVDVIINCT GVWAGALQAD ASLQPGRGQI IQVEAPWIKH FILTHDPSLG IYNSPYIIPG SKTVTLGGIF QLGNWSGLNS VRDHNTIWKS CCKLEPTLKN ARIVGELTGF RPVRPQVRLE REWLRHGSSS AEVIHNYGHG GYGLTIHWGC AMEAANLFGK ILEEKKLSRL PPSHL. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids. Reference: "Behavioral and biochemical characterization of a mutant mouse strain lacking D-amino acid oxidase activity and its implications for schizophrenia." Almond S.L., Fradley R.L., Armstrong E.J., Heavens R.B., Rutter A.R., Newman R.J., Chiu C.S., Konno R., Hutson P.H., Brandon N.J. Mol Cell Neurosci 32:324-334(2006). Function: nan