Cytotoxic T-lymphocyte Associated Protein 4, Recombinant, Guinea Pig, aa37-161, His-Tag (CTLA4)

Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. Source: Recombinant partial protein corresponding to aa37-161 of guinea pig Cytotoxic T-lymphocyte Associated Protein 4, fused to His-Tag at C-terminal, expressed in yeast. Molecular Weight: ~14.1kD, Biological Activity: The ED50 as determined by its ability to bind Mouse B7-1 in functional ELISA is less than 0.4ug/ml. Endotoxin: <1EU/ug (LAL). AA Sequence: AMHVAQPAVVLASSRGVASFECEYASSHNANEVRVTVLQQVASRTTEICAATYTVERELAFPEDSACAGTSSGTRVNLTIQGLRAADTGLYICKVELMYPPPYFVGTGNGTQIYVIDPEPCPDSD, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.