Cytotoxic and regulatory T-cell molecule (Crtam), partial (Active), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Mammalian cell. Expression Region: 17-289aa. Protein Length: Partial. Tag Info: C-terminal hFc-tagged. Target Protein Sequence: AFLKMETVTV EEGQTLTLTC VTSQTKNVSL QWLAPSGFTI FLNQHPALKS SKYQLLHHSA TQLSISVSNV TLREEGVYTC LHYGSSVKTK QVRVTVLVTP FQPTVEALVL RRQNGEKSVV LKCSTERSKP PPQITWLLGE GLEIYGELNH EFEADGKICN TSSMLIARAY GKNSTVHCII QHEGLHGRKL VAPFQFEDLV ADQETSDQET SDAPEQSSLS SQALQQPTST VSMMENSSIP ETDKEEKEHA TQDPGLSTEA SAQHTGLARR KSG. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Cadm1(CSB-MP004425MO) at 2 µg/mL can bind Mouse Crtam, the EC50 is 54.36 -61.77 ng/mL. Form: Lyophilized powder. Buffer: PBS, pH 7.4, 6% trehalose. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Mediates heterophilic cell-cell adhesion which regulates the activation, differentiation and tissue retention of various T-cell subsets. Interaction with CADM1 promotes natural killer (NK) cell cytotoxicity and IFNG/interferon-gamma secretion by CD8+ T-cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM1 in vivo.Interaction with CADM1 promotes natural killer (NK) cell cytotoxicity and IFNG/interferon-gamma secretion by CD8+ T-cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM1 in vivo. Reference: "A molecular analysis of NKT cells: identification of a class-I restricted T cell-associated molecule (CRTAM)." Kennedy J., Vicari A.P., Saylor V., Zurawski S.M., Copeland N.G., Gilbert D.J., Jenkins N.A., Zlotnik A. J. Leukoc. Biol. 67:725-734 (2000) "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Hayashizaki Y. Science 309:1559-1563 (2005). Function: nan