Cytosolic-abundant heat soluble protein 89226 (CAHS 89226), recombinant Hypsibius exemplaris

Organism: Hypsibius exemplaris (Freshwater tardigrade). Source: E.coli. Expression Region: 1-414aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: MATKESKYER VEKVNVDADG ATLVKNIGED RGKEDPGMNF QDKRPANLVP GAPAGVIPNR IESLPTDRAG QRLREHLSES ERLRVSRSST SSKSSSFVEP SLKYRGEIGP IGKNGEFVAS SNRQNSSSNV SSSDNSERAS PASRNSNPGM NNGMTTQRTT VITESSVQGL GAQRTVPIQP HQQREDHEVI THESHARAPE TTVVTIPTTR FESAQLESRR DGRTYTEDKE LTIPAPVVAP QIHAHQQVNM SGGTSATIHA TTDLHLASEA QINDMGPEEY ERYRAKVEAL ARIHEDETSR KAAAYRNAVE ADAELIRQTL ERQHMRDIEF RKDLVESSVD RQQQEIRLEA EYAMRALEQE RVNARAALDQ AMASTNIDVN IDSAIGTTHS QGRVTTTSES RTSQARGPAT AAVI. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: CAHS proteins are cytosolic heat soluble proteins that seem to contribute to the anhydrobiosis in tardigrades, but their specific mechanisms are yet to be identified. It is possible that protection during anhydrobiosis might occur via the stabilization of vitrifying small molecules such as sugars, but not via the direct glass transition of CAHS proteins themselves (Probable). Reference: n/a. Function: nan