Cytoplasmic dynein 2 light intermediate chain 1 (DYNC2LI1), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-352aa. Protein Length: Full Length of Isoform 2. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: MPSETLWEIA KAEVEKRGIN GSEGDGAEIA EKFVFFIGSK NGGKTTIILR CLDRDEPPKP TLALEYTYGR RAKGHNTPKD IAHFWELGGG TSLLDLISIP ITGDTLRTFS LVLVLDLSKP NDLWPTMENL LQATKSHVDK VIMKLGKTNA KAVSEMRQKI WNNMPKDHPQ DHELIDPFPV PLVIIGSKYD VFQDFESEKR KVICKTLRFV AHYYGASLMF TSKSEALLLK IRGVINQLAF GIDKSKSICV DQNKPLFITA GLDSFGQIGS PPVPENDIGK LHAHSPMELW KKVYEKLFPP KSINTLKDIK DPARDPQYAE NEVDEMRIQK DLELEQYKRS SSKSWKQIEL DS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May function as a motor for intraflagellar retrograde transport. Functions in cilia biogenesis (By similarity). Reference: "Identification of a novel light intermediate chain (D2LIC) for mammalian cytoplasmic dynein 2."Grissom P.M., Vaisberg E.A., McIntosh J.R.Mol. Biol. Cell 13:817-829(2002). Function: Required for correct intraflagellar transport (IFT), the bi-directional movement of particles required for the assembly, maintenance and functioning of primary cilia. Involved in the regulation of ciliary length.