Cytochrome P450 2J2 (CYP2J2), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 33-502aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: DFLKRRRPKN YPPGPWRLPF LGNFFLVDFE QSHLEVQLFV KKYGNLFSLE LGDISAVLIT GLPLIKEALI HMDQNFGNRP VTPMREHIFK KNGLIMSSGQ AWKEQRRFTL TALRNFGLGK KSLEERIQEE AQHLTEAIKE ENGQPFDPHF KINNAVSNII CSITFGERFE YQDSWFQQLL KLLDEVTYLE ASKTCQLYNV FPWIMKFLPG PHQTLFSNWK KLKLFVSHMI DKHRKDWNPA ETRDFIDAYL KEMSKHTGNP TSSFHEENLI CSTLDLFFAG TETTSTTLRW ALLYMALYPE IQEKVQAEID RVIGQGQQPS TAARESMPYT NAVIHEVQRM GNIIPLNVPR EVTVDTTLAG YHLPKGTMIL TNLTALHRDP TEWATPDTFN PDHFLENGQF KKREAFMPFS IGKRACLGEQ LARTELFIFF TSLMQKFTFR PPNNEKLSLK FRMGITISPV SHRLCAVPQV. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: A cytochrome P450 monooxygenase involved in the metabolism of polyunsaturated fatty acids (PUFA) in the cardiovascular system. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of PUFA. Converts arachidonic acid to four regioisomeric epoxyeicosatrienoic acids (EpETrE), likely playing a major role in the epoxidation of endogenous cardiac arachidonic acid pools. In endothelial cells, participates in eicosanoids metabolism by converting hydroperoxide species into hydroxy epoxy metabolites. In combination with 15-lipoxygenase metabolizes arachidonic acid and converts hydroperoxyicosatetraenoates (HpETEs) into hydroxy epoxy eicosatrienoates (HEETs), which are precursors of vasodilatory trihydroxyicosatrienoic acids (THETAs). This hydroperoxide isomerase activity is NADPH- and O2-independent. Catalyzes the monooxygenation of a various xenobiotics, such as danazol, amiodarone, terfenadine, astemizole, thioridazine, tamoxifen, cyclosporin A and nabumetone. Catalyzes hydroxylation of the anthelmintics albendazole and fenbendazole. Catalyzes the sulfoxidation of fenbedazole. Reference: n/a. Function: nan