Cytochrome P450 2E1 (Cyp2e1), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 2-493aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: AVLGITVALL VWIATLLLVS IWKQIYRSWN LPPGPFPIPF FGNIFQLDLK DIPKSLTKLA KRFGPVFTLH LGQRRIVVLH GYKAVKEVLL NHKNEFSGRG DIPVFQEYKN KGIIFNNGPT WKDVRRFSLS ILRDWGMGKQ GNEARIQREA HFLVEELKKT KGQPFDPTFL IGCAPCNVIA DILFNKRFDY DDKKCLELMS LFNENFYLLS TPWIQAYNYF SDYLQYLPGS HRKVMKNVSE IRQYTLGKAK EHLKSLDINC PRDVTDCLLI EMEKEKHSQE PMYTMENISV TLADLFFAGT ETTSTTLRYG LLILMKYPEI EEKLHEEIDR VIGPSRAPAV RDRMNMPYMD AVVHEIQRFI NLVPSNLPHE ATRDTVFRGY VIPKGTVVIP TLDSLLFDNY EFPDPETFKP EHFLNENGKF KYSDYFKAFS AGKRVCVGEG LARMELFLLL SAILQHFNLK SLVDPKDIDL SPVTIGFGSI PREFKLCVIP RS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics. Reference: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."The MGC Project Team Genome Res. 14:2121-2127(2004). Function: nan