CUB domain-containing protein 1 (Cdcp1), partial (Active), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Mammalian cell. Expression Region: 30-666aa. Protein Length: Partial. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: SEIALPQRSG VTVSIKLGNP ALPVKICYIV MSRQHITELI IRPGERKSFT FSCSNPEKHF VLKIEKNIDC MSGPCPFGEV HLQPSTSELP ILNRTFIWDV RAHKSIGLEL QFATPRLRQI GPGESCADGV THSISGHIDA TEVRIGTFCS NGTVSRIKMQ EGVKMALHLP WFHRRNVSGF SIANRSSIKR LCIIESVFEG EGSATLMSAN YPGGFPEDEL MTWQFVVPAH LRASVSFLNF NVSNCERKEE RVEYYIPGST TNPEVFRLED KQPGNMAGNF NLSLQGCDQD AQSPGILRLQ FQVLVQRPQD ESNKTYMVDL SRERTMSLTI EPRPVKHGRR FVPGCFVCLE SRTCSTNVTL TAGSIHKISF LCDDLTRLWV NVEKTLSCLD HRYCYRQSFK LQVPDYILQL PVQLHDFSWK LLVPKDKLSL MLVPGQKLQQ HTQERPCNTS FGYHVTSTTP GQDLYFGSFC SGGSIEKIQV KQNSSVTLRA YAPSFQQEVS KQGLIVSYTP YFKEEGIFTV TPDTKNKVYL RSPNWDRGLP ALSSVSWNIS VPSNQVACLT VLKERSGLAC QSGRAFMIIQ EQQSRAEEIF SLEEEVLPKP SFHHHSFWVN ISNCSPMNGK QLDLLFWVTL TPRTVDL. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Cdcp1 at 2 µg/mL can bind Anti-CDCP1 recombinant antibody (CSB-RA884474MA1HU), the EC50 is 0.6397-0.8369 ng/ml. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis. Reference: "CDCP1 on Dendritic Cells Contributes to the Development of a Model of Kawasaki Disease."Lun Y., Borjini N., Miura N.N., Ohno N., Singer N.G., Lin F.J Immunol 206:2819-2827 (2021). Function: nan