Complement C1s-1 subcomponent (C1s1), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 16-688aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: EPTMHGEILS PNYPQAYPND VVKSWDIEVP EGFGIHLYFT HVDIEPSESC AYDSVQIISG GIEEGRLCGQ KTSKSPNSPI IEEFQFPYNK LQVVFTSDFS NEERFTGFAA YYTAIDINEC TDFTDVPCSH FCNNFIGGYF CSCPPEYFLH DDMRNCGVNC SGDVFTALIG EISSPNYPNP YPENSRCEYQ IQLQEGFQVV VTMQREDFDV EPADSEGNCP DSLTFASKNQ QFGPYCGNGF PGPLTIRTQS NTLGIVFQTD LMGQKKGWKL RYHGDPISCA KKITANSTWE PDKAKYVFKD VVKITCVDGF EVVEGHVSST SYYSTCQSDG QWSNSGLKCQ PVYCGIPDPI ANGKVEEPEN SVFGTVVHYT CEEPYYYMEH EEGGEYRCAA NGRWVNDQLG IELPRCIPAC GVPTEPFQVH QRIFGGQPAK IENFPWQVFF NHPRASGALI NEYWVLTAAH VLEKISDPLM YVGTMSVRTT LLENAQRLYS KRVFIHPSWK KEDDPNTRTN FDNDIALVQL KDPVKMGPKV SPICLPGTSS EYNVSPGDMG LISGWGSTEK KVFVINLRGA KVPVTSLETC KQVKEENPTV RPEDYVFTDN MICAGEKGVD SCHGDSGGAF AFQVPNVTVP KFYVAGLVSW GKRCGTYGVY TKVKNYVDWI LKTMQENSGP RKD. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: C1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4. Reference: "Complement C1r and C1s genes are duplicated in the mouse: differential expression generates alternative isomorphs in the liver and in the male reproductive system." Garnier G., Circolo A., Xu Y., Volanakis J.E. Biochem. J. 371:631-640(2003). Function: nan