COMM domain-containing protein 5 (COMMD5), human, recombinant

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 2-224aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: SAVGAATPYL HHPGDSHSGR VSFLGAQLPP EVAAMARLLG DLDRSTFRKL LKFVVSSLQG EDCREAVQRL GVSANLPEEQ LGALLAGMHT LLQQALRLPP TSLKPDTFRD QLQELCIPQD LVGDLASVVF GSQRPLLDSV AQQQGAWLPH VADFRWRVDV AISTSALARS LQPSVLMQLK LSDGSAYRFE VPTAKFQELR YSVALVLKEM ADLEKRCERR LQD. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May modulate activity of cullin-RING E3 ubiquitin ligase (CRL) complexes . Negatively regulates cell proliferation. Negatively regulates cell cycle G2/M phase transition probably by transactivating p21/CDKN1A through the p53/TP53-independent signaling pathway. Involved in kidney proximal tubule morphogenesis. Down-regulates activation of NF-kappa-B . Reference: "COMMD1 (copper metabolism MURR1 domain-containing protein 1) regulates Cullin RING ligases by preventing CAND1 (Cullin-associated Nedd8-dissociated protein 1) binding." Mao X., Gluck N., Chen B., Starokadomskyy P., Li H., Maine G.N., Burstein E. J. Biol. Chem. 286:32355-32365(2011). Function: nan