Collagen alpha-3 (IV) chain (Col4a3), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 1424-1669aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: PGLKGNPGDR GTPATGTRMR GFIFTRHSQT TAIPSCPEGT QPLYSGFSLL FVQGNKRAHG QDLGTLGSCL QRFTTMPFLF CNINNVCNFA SRNDYSYWLS TPALMPMDMA PISGRALEPY ISRCTVCEGP AMAIAVHSQT TAIPPCPQDW VSLWKGFSFI MFTSAGSEGA GQALASPGSC LEEFRASPFI ECHGRGTCNY YSNSYSFWLA SLNPERMFRK PIPSTVKAGD LEKIISRCQV CMKKRH. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Tumstatin, a cleavage fragment corresponding to the collagen alpha 3(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity, these two anti-tumor properties may be regulated via RGD-independent ITGB3-mediated mechanisms. Reference: "Physiological levels of tumstatin, a fragment of collagen IV alpha3 chain, are generated by MMP-9 proteolysis and suppress angiogenesis via alphaV beta3 integrin." Hamano Y., Zeisberg M., Sugimoto H., Lively J.C., Maeshima Y., Yang C., Hynes R.O., Werb Z., Sudhakar A., Kalluri R. Cancer Cell 3:589-601(2003). Function: nan