Collagen alpha-3 (IV) chain (COL4A3), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1427-1668aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: GLKGKRGDSG SPATWTTRGF VFTRHSQTTA IPSCPEGTVP LYSGFSFLFV QGNQRAHGQD LGTLGSCLQR FTTMPFLFCN VNDVCNFASR NDYSYWLSTP ALMPMNMAPI TGRALEPYIS RCTVCEGPAI AIAVHSQTTD IPPCPHGWIS LWKGFSFIMF TSAGSEGTGQ ALASPGSCLE EFRASPFLEC HGRGTCNYYS NSYSFWLASL NPERMFRKPI PSTVKAGELE KIISRCQVCM KK. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Type IV collagen is the major structural component of glomerular basent mbranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.Tumstatin, a cleavage fragment corresponding to the collagen alpha 3(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity, these two anti-tumor properties may be regulated via RGD-independent ITGB3-mediated mechanisms. Reference: Novel COL4A5, COL4A4, and COL4A3 mutations in Alport syndrome.Nagel M., Nagorka S., Gross O.Hum. Mutat. 26:60-60(2005). Function: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen., FUNCTION