Colicin-E1 (cea), Escherichia coli, recombinant

Organism: Escherichia coli. Source: in vitro E.coli expression system. Expression Region: 1-522aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: METAVAYYKD GVPYDDKGQV IITLLNGTPD GSGSGGGGGK GGSKSESSAA IHATAKWSTA QLKKTQAEQA ARAKAAAEAQ AKAKANRDAL TQRLKDIVNE ALRHNASRTP SATELAHANN AAMQAEDERL RLAKAEEKAR KEAEAAEKAF QEAEQRRKEI EREKAETERQ LKLAEAEEKR LAALSEEAKA VEIAQKKLSA AQSEVVKMDG EIKTLNSRLS SSIHARDAEM KTLAGKRNEL AQASAKYKEL DELVKKLSPR ANDPLQNRPF FEATRRRVGA GKIREEKQKQ VTASETRINR INADITQIQK AISQVSNNRN AGIARVHEAE ENLKKAQNNL LNSQIKDAVD ATVSFYQTLT EKYGEKYSKM AQELADKSKG KKIGNVNEAL AAFEKYKDVL NKKFSKADRD AIFNALASVK YDDWAKHLDQ FAKYLKITGH VSFGYDVVSD ILKIKDTGDW KPLFLTLEKK AADAGVSYVV ALLFSLLAGT TLGIWGIAIV TGILCSYIDK NKLNTINEVL GI. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: This colicin is a channel-forming colicin. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy. Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria. Reference: "Nucleotide polymorphism in colicin E1 and Ia plasmids from natural isolates of Escherichia coli." Riley M.A., Tan Y., Wang J. Proc. Natl. Acad. Sci. U.S.A. 91:11276-11280(1994). Function: This colicin is a channel-forming colicin. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy., FUNCTION