Cocaine esterase (CES2), recombinant human

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 27-559aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: QDSASPIRTT HTGQVLGSLV HVKGANAGVQ TFLGIPFAKP PLGPLRFAPP EPPESWSGVR DGTTHPAMCL QDLTAVESEF LSQFNMTFPS DSMSEDCLYL SIYTPAHSHE GSNLPVMVWI HGGALVFGMA SLYDGSMLAA LENVVVVIIQ YRLGVLGFFS TGDKHATGNW GYLDQVAALR WVQQNIAHFG GNPDRVTIFG ESAGGTSVSS LVVSPISQGL FHGAIMESGV ALLPGLIASS ADVISTVVAN LSACDQVDSE ALVGCLRGKS KEEILAINKP FKMIPGVVDG VFLPRHPQEL LASADFQPVP SIVGVNNNEF GWLIPKVMRI YDTQKEMDRE ASQAALQKML TLLMLPPTFG DLLREEYIGD NGDPQTLQAQ FQEMMADSMF VIPALQVAHF QCSRAPVYFY EFQHQPSWLK NIRPPHMKAD HGDELPFVFR SFFGGNYIKF TEEEEQLSRK MMKYWANFAR NGNPNGEGLP HWPLFDQEEQ YLQLNLQPAV GRALKAHRLQ FWKKALPQKI QELEEPEERH TEL. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: n/a. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered PBS, 6% Trehalose, pH 7.4. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows high catalytic efficiency for hydrolysis of cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine. Hydrolyzes aspirin, substrates with large alcohol group and small acyl group and endogenous lipids such as triacylglycerol. Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes of 2-arachidonoylglycerol and prostaglandins. Reference: Complete sequencing and characterization of 21,243 full-length human cDNAs. Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Sugano S. Nat. Genet. 36:40-45 (2004). Function: nan