Citrullinated GRP78 (human recombinant)

Item number Size Datasheet Manual SDS Delivery time Quantity Price
Cay25107-100 100 µg -

6 - 10 business days*

730.00€
 
Glucose-regulated protein 78 kDa (GRP78) is a molecular chaperone that is ubiquitously expressed... more
Product information "Citrullinated GRP78 (human recombinant)"
Glucose-regulated protein 78 kDa (GRP78) is a molecular chaperone that is ubiquitously expressed in the endoplasmic reticulum of mammalian cells. GRP78 can be citrullinated at its 27 arginine residues by protein deiminases (PADs). The accumulation of citrullinated proteins in vivo leads to the production of anti-citrullinated protein antibodies (ACPAs) which perpetuate the inflammatory process. In vitro, ACPAs bind to citrullinated GRP78 expressed on the cell surface of peripheral blood mononuclear cells PMBCs and U937 cells leading to the production of TNF-alpha. In a mouse model of collagen-induced arthritis (CIA), anti-citrullinated GRP78 antibodies are found in the serum. Pre-immunization with citrullinated GRP78 prior to CIA induction shortens the time to joint inflammation and increases arthritis scores compared with non-citrullinated GRP78-immunized and non-immunized control mice. In autoimmune diseases such as rheumatoid arthritis, patient-derived serum contains higher levels of anti-citrullinated GRP78 antibodies than serum derived from patients with systemic lupus erythematosus and healthy controls.Synonyms: BiP, Endoplasmic Reticulum Lumenal Ca2+-Binding Protein GRP78, Glucose-Related Protein 78, Heat Shock Protein 5 (70 kDa), Hsp5 (70 kDa), Immunoglobulin Heavy Chain-Binding Protein. Purity: >90% estimated by SDS-PAGE. Source: N-Terminal histidine-tagged human GRP78 purified from E. coli, citrullinated by PAD2. Amino Acids: 2-654. MW: 74.6 kDa. Storage Buffer: PBS, pH 7.4, with 10% glycerol. UniProt Accession #: P11021.
Keywords: BiP {ECO:0000303|Ref.4}, BiP {ECO:0000303|Ref.4}, GRP-78 {ECO:0000303|PubMed:2840249}, GRP-78 {ECO:0000303|PubMed:2840249}, HSP70 family protein 5 {ECO:0000305}, HSP70 family protein 5 {ECO:0000305}, Endoplasmic reticulum chaperone BiP {ECO:0000305}, Endo
Supplier: Cayman Chemical
Supplier-Nr: 25107

Properties

Application: WB control
Conjugate: No
Host: E.coli
Species reactivity: human
MW: 74.6 kD
Purity: >90% estimated by SDS-PAGE
Format: Purified

Handling & Safety

Storage: -80°C
Shipping: -80°C (International: -80°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
You will get a certificate here
or to request a certificate of analysis.
Read, write and discuss reviews... more
Customer review for "Citrullinated GRP78 (human recombinant)"
Write a review
or to review a product.
Viewed