Chymotrypsin-like protease VLCTLP, Macrovipera lebetina, recombinant

Organism: Macrovipera lebetina (Levantine viper) (Vipera lebetina). Source: E.coli. Expression Region: 25-257aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: VVGGDECNIN EHRSLVFLYN SSFGCGGTLI NQQWVLSAAH CDMENVQIYL GLHNLRLRNQ DEQIRVAEEK FFCLSNKSYT KWDKDIMLIR LNSSVTYNTH IAPLSLPSSP PRVGSVCRIM GWGAITSPNE TFPNVPHCAN INILRYSVCR AAYRGLPAQS RTLCAGILQG GIGSCMGDSG GPLICNGEIQ GIVSWGDDIC AQPHKPVHYT KVFDYSDWIQ SIIAGNTTAT CPL. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: Not Test. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Snake venom serine protease with tyrosine-specific chymotrypsin-like activity. Hydrolyzes the N-acetyl-L-tyrosine ethyl ester (ATEE). Has weak fibrinogenolytic activity. Weakly hydrolyzes azocasein, Aalpha-chain (FGA) and more slowly Bbeta-chain (FGB) of fibrinogen. Optimal substrates are angiotensins I and II (AGT). Reference: n/a. Function: nan