Chondroitin sulfate proteoglycan 4 (Cspg4), partial, rat, recombinant

Organism: Rattus norvegicus (Rat). Source: E.coli. Expression Region: 575-1044aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: GPEIFQAYDP DSACEGLTIQ LLGVSASVPV EHRDQPGEPV TEFSCRDLEA GNIVYVHRGG PAQDLTFRVS DGMQASGPAT LKVVAVRPAI QILHNTGLRL AQGSAAAILP ANLSVETNAV GQDVSVLFRV TGTLQFGELQ KQGAGGVEGT EWWDTLAFHQ RDVEQGRVRY LSTDPQHHTQ DTVEDLTLEV QVGQETLSNL SFPVTIQRAT VWMLQLEPLH TQNPHQETLT SAHLEASLEE EGEGGPYPHI FHYELVQAPR RGNLLLQGTR LSDGQSFSQS DLQAGRVTYR ATTRTSEAAE DSFRFRVTSP PHFSPLYTFP IHIGGDPNAP VLTNVLLMVP EGGEGVLSAD HLFVKSLNSA SYLYEVMEQP HHGSLAWRDP KGRATPVTSF TNEDLLHGRL VYQHDDSETI EDDIPFVATR QGEGSGDMAW EEVRGVFRVA IQPVNDHAPV QTISRVFHVA RGGQRLLTTD. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades. Reference: "Phosphorylation of NG2 proteoglycan by protein kinase C-alpha regulates polarized membrane distribution and cell motility." Makagiansar I.T., Williams S., Dahlin-Huppe K., Fukushi J., Mustelin T., Stallcup W.B. J. Biol. Chem. 279:55262-55270(2004). Function: nan