Chondroitin sulfate proteoglycan 4 (CSPG4), partial, human, recombinant

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 1583-2224aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: SLKGSQTLTV CPGSVQPLSS QTLRASSSAG TDPQLLLYRV VRGPQLGRLF HAQQDSTGEA LVNFTQAEVY AGNILYEHEM PPEPFWEAHD TLELQLSSPP ARDVAATLAV AVSFEAACPQ RPSHLWKNKG LWVPEGQRAR ITVAALDASN LLASVPSPQR SEHDVLFQVT QFPSRGQLLV SEEPLHAGQP HFLQSQLAAG QLVYAHGGGG TQQDGFHFRA HLQGPAGASV AGPQTSEAFA ITVRDVNERP PQPQASVPLR LTRGSRAPIS RAQLSVVDPD SAPGEIEYEV QRAPHNGFLS LVGGGLGPVT RFTQADVDSG RLAFVANGSS VAGIFQLSMS DGASPPLPMS LAVDILPSAI EVQLRAPLEV PQALGRSSLS QQQLRVVSDR EEPEAAYRLI QGPQYGHLLV GGRPTSAFSQ FQIDQGEVVF AFTNFSSSHD HFRVLALARG VNASAVVNVT VRALLHVWAG GPWPQGATLR LDPTVLDAGE LANRTGSVPR FRLLEGPRHG RVVRVPRART EPGGSQLVEQ FTQQDLEDGR LGLEVGRPEG RAPGPAGDSL TLELWAQGVP PAVASLDFAT EPYNAARPYS VALLSVPEAA RTEAGKPESS TPTGEPGPMA SSPEPAVAKG GFLSFLEANM FS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2 which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades. Reference: "A role of chondroitin sulfate glycosaminoglycan binding site in alpha4beta1 integrin-mediated melanoma cell adhesion." Iida J., Meijne A.M.L., Oegema T.R. Jr., Yednock T.A., Kovach N.L., Furcht L.T., McCarthy J.B. J. Biol. Chem. 273:5955-5962(1998). Function: nan