Chondroitin AC lyase 8A, Bacteroides stercoris

Chondroitin AC lyase 8A (BsCac8A), assigned the E.C. number 4.2.2.5, is a derivative of Bacteroides stercoris. It is an enzyme that participates in the eliminative degradation of polysaccharides containing 1,4-beta-D-hexosaminyl and 1,3-beta-D-glucuronosyl linkages to disaccharides containing 4-deoxy-beta-D-gluc-4-enuronosyl groupsanhydrobond in the MurNAc residue. The recombinant BsCac8A, purified from Escherichia coli, is a single-domain Pectate Lyase family 8 (PL8) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 0,25 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Polysaccharide Lyase. Activity: Chondroitin lyases. Family: PL8. Ec Number: 4.2.2.5. Organism: Bacteroides stercoris. Architecture: PL8. Optimal pH: 5.8. Optimal Temperature: 45°C. Substrates: chondroitin sulfate A (CS-A), chondroitin sulfate C (CS-C), hyaluronic acid (hyaluronan)